Composite
Part:BBa_K3384136:Design
Designed by: Yiqing Zhang Group: iGEM20_NJTech_China (2020-10-26)
pprm1 Pro-GFP-CYC1 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1123
Design Notes
Please visit to the design of the basic part BBa_K3384313 for the construction process of pprm1 Pro. The green fluorescent protein (GFP) was obtained by PCR amplification using engineered Saccharomyces cerevisiae BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.
Source
Saccharomyces cerevisiae